human total mmp 1 duoset Search Results


mmp  (R&D Systems)
95
R&D Systems mmp
Mmp, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mmp - by Bioz Stars, 2026-02
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mmp 1 duoset  (R&D Systems)
94
R&D Systems mmp 1 duoset
CSE-induced CCN1 augments matrix metalloproteinase <t>(MMP)-1</t> secretion. A: effects of CCN1 knockdown on MMP1. Beas2B cells were treated with control or CCN1 siRNA, followed with 10% CSE. MMP1 levels in supernatants were determined using ELISA 4 h later. B: the soluble factor and extracellular vehicles (EVs) were isolated from CSE-stimulated Beas2B cells. Beas2B cells were treated with control or CCN1 siRNA. The Beas2B cells were stimulated by the soluble factor (10 μg) and EV (10 μg). MMP1 was then determined using ELISA. C: Beas2B cells were treated with recombinant CCN1 (1 μg/ml), plasmin (0.1 μg/ml), and CCN1 + plasmin. After 8 h, MMP1 was determined using ELISA. CCN1 and plasmin were preincubated 1 h at 37°C before adding to the cells. D: Beas2B cells were pretreated for 1 h with TLCK before CSE, and MMP1 was determined using ELISA 8 h later. E: level of MMP1 after treatment of NH2-terminal or COOH-terminal CCN1 fragments (each 1 μg) in Beas2B cells. Supernatant (Sup) was taken after 24 h and measured MMP1. F: cell survival after treatment of NH2-terminal or COOH-terminal CCN1 fragments for 48 h in Beas2B cells. Cell survival was measured by CellTiter-Glo Luminescent cell viability assay. *P < 0.05 compared with control for A and B, sham for C and F, and CSE for D and E. **P < 0.05 compared with CCN1 and plasmin for C and NH2-terminal for E. Each panel represents at least 3 repeats with similar results.
Mmp 1 Duoset, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mmp 1 duoset/product/R&D Systems
Average 94 stars, based on 1 article reviews
mmp 1 duoset - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier

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CSE-induced CCN1 augments matrix metalloproteinase (MMP)-1 secretion. A: effects of CCN1 knockdown on MMP1. Beas2B cells were treated with control or CCN1 siRNA, followed with 10% CSE. MMP1 levels in supernatants were determined using ELISA 4 h later. B: the soluble factor and extracellular vehicles (EVs) were isolated from CSE-stimulated Beas2B cells. Beas2B cells were treated with control or CCN1 siRNA. The Beas2B cells were stimulated by the soluble factor (10 μg) and EV (10 μg). MMP1 was then determined using ELISA. C: Beas2B cells were treated with recombinant CCN1 (1 μg/ml), plasmin (0.1 μg/ml), and CCN1 + plasmin. After 8 h, MMP1 was determined using ELISA. CCN1 and plasmin were preincubated 1 h at 37°C before adding to the cells. D: Beas2B cells were pretreated for 1 h with TLCK before CSE, and MMP1 was determined using ELISA 8 h later. E: level of MMP1 after treatment of NH2-terminal or COOH-terminal CCN1 fragments (each 1 μg) in Beas2B cells. Supernatant (Sup) was taken after 24 h and measured MMP1. F: cell survival after treatment of NH2-terminal or COOH-terminal CCN1 fragments for 48 h in Beas2B cells. Cell survival was measured by CellTiter-Glo Luminescent cell viability assay. *P < 0.05 compared with control for A and B, sham for C and F, and CSE for D and E. **P < 0.05 compared with CCN1 and plasmin for C and NH2-terminal for E. Each panel represents at least 3 repeats with similar results.

Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

Article Title: CCN1 secretion and cleavage regulate the lung epithelial cell functions after cigarette smoke

doi: 10.1152/ajplung.00102.2014

Figure Lengend Snippet: CSE-induced CCN1 augments matrix metalloproteinase (MMP)-1 secretion. A: effects of CCN1 knockdown on MMP1. Beas2B cells were treated with control or CCN1 siRNA, followed with 10% CSE. MMP1 levels in supernatants were determined using ELISA 4 h later. B: the soluble factor and extracellular vehicles (EVs) were isolated from CSE-stimulated Beas2B cells. Beas2B cells were treated with control or CCN1 siRNA. The Beas2B cells were stimulated by the soluble factor (10 μg) and EV (10 μg). MMP1 was then determined using ELISA. C: Beas2B cells were treated with recombinant CCN1 (1 μg/ml), plasmin (0.1 μg/ml), and CCN1 + plasmin. After 8 h, MMP1 was determined using ELISA. CCN1 and plasmin were preincubated 1 h at 37°C before adding to the cells. D: Beas2B cells were pretreated for 1 h with TLCK before CSE, and MMP1 was determined using ELISA 8 h later. E: level of MMP1 after treatment of NH2-terminal or COOH-terminal CCN1 fragments (each 1 μg) in Beas2B cells. Supernatant (Sup) was taken after 24 h and measured MMP1. F: cell survival after treatment of NH2-terminal or COOH-terminal CCN1 fragments for 48 h in Beas2B cells. Cell survival was measured by CellTiter-Glo Luminescent cell viability assay. *P < 0.05 compared with control for A and B, sham for C and F, and CSE for D and E. **P < 0.05 compared with CCN1 and plasmin for C and NH2-terminal for E. Each panel represents at least 3 repeats with similar results.

Article Snippet: The human IL-8 ELISA kit was purchased from Thermo (Rockford, IL), and human VEGF and MMP-1 duoset was from R&D Systems and followed the manufacturer's instructions. .

Techniques: Enzyme-linked Immunosorbent Assay, Isolation, Recombinant, Cell Viability Assay